The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. The authors declare that they have no competing interests. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Dextran. government site. Accessibility International Journal of Pharmaceutics, 277, 99-104. Dextrans in Aqueous Solution. Experimental Review on Intrinsic ; Yevlampieva N.P. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Figure5 shows the cumulative amount of the FITC-dextran (M.W. Eremeeva, T. E.; Bykova, T. O. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Experimental and numerical study on the mixed lubrication For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. HHS Vulnerability Disclosure, Help Dynamics of a Polymer Solution in a Rigid Matriz. 8. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). The Hydrodynamic Radii of Macromolecules and Their PubMedGoogle Scholar. Springer Nature. Soc., 16, 3A, 426-433. MeSH terms Viswanath, D.S. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Indo AM J Pharm. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Trop J Pharm Res. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Arch Dermatol Res. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. "Dextrans in Aqueous Solution. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. 2018;1:10915. Webof overall macromolecular geometry and hydrodynamic draining. eCollection 2018 Feb. Molecules. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. Biophysical Journal, 27, 177-181. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Dextran The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. The https:// ensures that you are connecting to the Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Regulating dynamic signaling between hematopoietic stem cells Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. WebFITC Products. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. 2010;4(1):117. Epub 2022 Feb 2. Expert Opin Drug Deliv. Chem. Keywords: FITC dextran; intestinal permeability; transit time. Bookshelf They concluded that a power law behavior seems to be approached only in the small molecular weight region. 2011;5(2):95132. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Hydrodynamic Properties of Tragacanthin. (2007). HSE and DK helped to porate the skin and to measure TEWL. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). NCI CPTC Antibody Characterization Program. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! (1987). Rheology of dextran solutions. Biomaterials, 22, 1891-1898. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. WebR H Hydrodynamic radius [m] The basic DLS setup. It is well tolerated on parenteral infusion by most people but a very low incidence Progress in Polymer Science, 30, 38-70. European Polymer Journal, 42, 1086-1092. 2016;47:15964. The site is secure. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Hrabchak C, Flynn L, Woodhouse KA. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Sci. SWC designed and coordinated the research. (2012). The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. FITC-dextran solution (600L) was added to the donor chamber. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. J Pharm Sci. The human and pig skin permeability of FITC-dextran (M.W. Data File Dextran. dextran hydrodynamic radius. Pharm Res. Chem. Influence of molecular weight on Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Google Scholar. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Search Carbohydrate Polymers 88 373-381. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Protein Hydrodynamics with Light Scattering Carbohydrate Polymers 87, 405-410. Carbohydrate Polymers, 78, 902-907. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Journal of Applied Polymer Science, 27, 795-810. J Appl Toxicol. Global hydrodynamic analysis of the molecular flexibility of galactomannans. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) Gums and stabilisers for the Food Industry, 5, 447-450. ; Aberle, T; Burchard, W. (2000). Part of In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. ; Patel, T.R. Principles of Size Exclusion Chromatography SEC (GFC J Cosmet Laser Ther. 2005;304(1):27486. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence 2012;29(7):201729. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. However, in vitro investigations are very few. Fixable: Fixable via Lysine. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). ; Talsma, H.; Hennink, W.E. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. The skin poration was performed using the microporation device (LG electronics, Korea). The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Dextrans in Aqueous Solution. Experimental Review on Intrinsic dextran Microporation was performed 2, 5, and 10 times on each sample. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Int J Food Sci Nutr. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Weissella: An Emerging Bacterium with Promising Health Benefits. WebTry out PMC Labs and tell us what you think. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Table 2 compares the properties of nanoparticles coated with one or other polymer. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Springer Editions. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. Please enable it to take advantage of the complete set of features! There is a deviation from linearity after 580kDa [69, 70]. Webdextran hydrodynamic radius. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). 2011;16(2):518. The term f/f0 is sometimes denoted as P, Perrin constant. (2000). Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Front Microbiol. Naik A, Kalia YN, Guy RH. Polymers | Free Full-Text | Confinement-Induced Fractionation and Unable to load your collection due to an error, Unable to load your delegates due to an error. Nanoparticles in magnetic resonance imaging: from simple to dual For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. Hydrodynamic radii ( RH) were determined by dynamic light scattering. De Belder, A. N. (2001). The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. An official website of the United States government. Granath, K. A. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Hydrodynamic CAS National Library of Medicine Hindered diffusion of dextran and ficoll in microporous membranes. Biophysical Journal, 87, 4259-4270. 10K, and c M.W. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. CAS We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Appl Biochem Biotechnol. Dextrans can function efficiently as anterograde or retrograde tracers, Peptide and protein transdermal drug delivery. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Polysaccharides-based nanoparticles as drug delivery systems. Small Intestinal Permeability and Gut-Transit Time Determined Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. 20K groups (n=3, *: p<0.05, **: p<0.01). H&E stained images were analyzed using Image J software. Chemical penetration enhancers for transdermal drug delivery systems. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Carbohydrate Polymers, 68, 477-488. statement and WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), ; Fran, H.P. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Differences were considered statistically significant at p <0.05. Journal of Applied Polymer Science, 13, 2031-2032. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). The hydrodynamic 57 radius of the dextranase is 7 a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Dextrans in Aqueous Solution. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. Carbohydrate Research, 160, 205-214. Advanced Drug Delivery Reviews, 60, 1650-1662. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. J Control Release. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. (1999). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Int. Guner, Ali (1999). Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). American Journal of Food Science and Technology 2013, 1(3), 60-66. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. (2007). Barshtein, G.; Tamir, I.; Yedgar, S. (1998). Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. Characterisation of insulin analogues therapeutically available to Advances in Carbohydrate Analysis, 1, 63-144. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. ; Rjumtsev, E.I. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. Polysaccharide hydrogels for modified release formulations. We observe that this maximum radius is below For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. If there are particles in the sample, the incident laser light gets scattered in all directions. On the molecular weight distribution of dextran T-500. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. The unfolding of iRFP713 in a crowded milieu - PeerJ Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g).